Wu, Hao published the artcileMicroarray-Assisted High-Throughput Identification of a Cell-Permeable Small-Molecule Binder of 14-3-3 Proteins, Application of 4-Fluoro-2-methylbenzene-1-sulfonyl chloride, the main research area is cell permeable peptide library preparation microarray screening protein binding.
The authors have used a small-mol. microarray (SMM) technique to identify cell-permeable small mol.-peptide hybrids that are capable of binding to all 14-3-3 proteins and thus inhibiting protein-protein interactions (PPI). Phosphoserine-containing peptide libraries were constructed on solid-phase using 243 different carboxylic acids for conjugation with N-terminus of individual peptides in one library, and 50 different amines for conjugation with C-terminus of individual peptides in the second library. For both libraries, biotin and GlyGly linkers were introduced at either N- or C-terminus of each peptide to facilitate subsequent immobilization onto avidin-functionalized glass slides. All the peptide conjugates were characterized by liquid chromatog./mass spectrometry and most were shown to be the correct mol. weight and of sufficient purity for direct microarray applications.
Angewandte Chemie, International Edition published new progress about 14-3-3 Proteins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 7079-48-3 belongs to class chlorides-buliding-blocks, name is 4-Fluoro-2-methylbenzene-1-sulfonyl chloride, and the molecular formula is C7H6ClFO2S, Application of 4-Fluoro-2-methylbenzene-1-sulfonyl chloride.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics