Tag: 300-400

Disinfectant and antimicrobial susceptibility studies of the foodborne pathogen Campylobacter jejuni isolated from the litter of broiler chicken houses was written by Beier, Ross C.;Byrd, J. Allen;Andrews, Kathleen;Caldwell, Denise;Crippen, Tawni L.;Anderson, Robin C.;Nisbet, David J.. And the article was included in Poultry Science in 2021.SDS of cas: 122-18-9 The following contents are mentioned in the article:

Foodborne illness is an ongoing problem worldwide and is caused by bacteria that invade the food chain from the farm, slaughter house, restaurant or grocery, or in the home and can be controlled by strategies using biocides (antiseptics and disinfectants). Susceptibility profiles were determined for 96 Campylobacter jejuni strains obtained in 2011-2012 from broiler chicken house environments to antimicrobials and disinfectants as per the methods of the Clin. and Laboratory Standards Institute and TREK Diagnostics using CAMPY AST Campylobacter plates. Low prevalence of antimicrobial resistance was observed in C. jejuni strains to tetracycline (TET; 21.9%), ciprofloxacin (CIP; 13.5%), and nalidixic acid (NAL; 12.5%). The resistance profiles had a maximum of 3 antimicrobials, CIP-NAL-TET, with TET being the main profile observed No cross-resistance was observed between antimicrobials and disinfectants. The C. jejuni strains (99%) were resistant to triclosan, 32% were resistant to chlorhexidine, and they all were susceptible to benzalkonium chloride. The strains had low-level min. inhibitory concentrations (MICs) to the disinfectants P-128, Food Service Sanitizer, F-25 Sanitizer, Final Step 512 Sanitizer, OdoBan, dioctyldimethylammmonium chloride, didecyldimethylammonium chloride (C10AC), benzyldimethyldodecylammonium chloride (C12BAC), and benzyldimethyltetradecylammonium chloride (C14BAC). Intermediate MICs against DC&R, cetylpyridinium bromide hydrate, hexadecylpyridinium chloride, ethylhexadecyldimethylammonium bromide, and hexadecyltrimethylammonium bromide with elevated intermediate MICs against Tek-Trol, benzyldimethylhexadecylammonium chloride, tris(hydroxylmethyl)nitromethane (THN), and formaldehyde. The highest MIC were obtained for povidone-iodine. The components THN and the benzylammonium chlorides C12BAC and C14BAC were responsible for the inhibition by DC&R. The components C10AC and C12BAC may act synergistically causing inhibition of C. jejuni by the disinfectant P-128. The formaldehyde component in DC&R was not effective against C. jejuni compared with the ammonium chloride components. Its use in disinfectants may result in addnl. unnecessary chems. in the environment. Didecyldimethylammonium chloride is the most effective ammonium chloride component against C. jejuni. This study involved multiple reactions and reactants, such as N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9SDS of cas: 122-18-9).

N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9) belongs to organic chlorides. Organic chlorides can cause corrosion in pipelines, valves and condensers, and cause catalyst poisoning. The hydrocarbon processing industry (HPI) and others are affected by damage caused by these substances. Aliphatic organochlorides are often alkylating agents as chlorine can act as a leaving group, which can result in cellular damage.SDS of cas: 122-18-9

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Liquid chromatography-tandem mass spectrometry multiresidue method for the analysis of quaternary ammonium compounds in cheese and milk products: Development and validation using the total error approach was written by Slimani, Kahina;Feret, Aurelie;Pirotais, Yvette;Maris, Pierre;Abjean, Jean-Pierre;Hurtaud-Pessel, Dominique. And the article was included in Journal of Chromatography A in 2017.COA of Formula: C25H46ClN The following contents are mentioned in the article:

Quaternary ammonium compounds (QACs) are both cationic surfactants and biocidal substances widely used as disinfectants in the food industry. A sensitive and reliable method for the anal. of benzalkonium chlorides (BACs) and dialkyldimethylammonium chlorides (DDACs) has been developed that enables the simultaneous quant. determination of ten quaternary ammonium residues in dairy products below the provisional maximum residue level (MRL), set at 0.1 mg kg^-1. To the best of our knowledge, this method could be the one applicable to milk and to three major processed milk products selected, namely processed or hard pressed cheeses, and whole milk powder. The method comprises solvent extraction using a mixture of acetonitrile and Et acetate, without any further clean-up. Analyses were performed by liquid chromatog. coupled with electrospray tandem mass spectrometry detection (LC-ESI-MS/MS) operating in pos. mode. A C18 anal. column was used for chromatog. separation, with a mobile phase composed of acetonitrile and water both containing 0.3% formic acid; and methanol in the gradient mode. Five deuterated internal standards were added to obtain the most accurate quantification. Extraction recoveries were satisfactory and no matrix effects were observed The method was validated using the total error approach in accordance with the NF V03-110 standard in order to characterize the trueness, repeatability, intermediate precision and anal. limits within the range of 5-150 μg kg^-1 for all matrixes. These performance criteria, calculated by e.noval 3.0 software, were satisfactory and in full accordance with the proposed provisional MRL and with the recommendations in the European Union SANTE/11945/2015 regulatory guidelines. The limit of detection (LOD) was low (<1.9 μg kg^-1) and the limit of quantification (LOQ) ranged from 5 μg kg^-1 to 35 μg kg^-1 for all matrixes depending on the analytes. The validation results proved that the method is suitable for quantifying quaternary ammoniums in foodstuffs from dairy industries at residue levels, and could be used for biocide residues monitoring plans and to measure the exposition consumer to biocides products. This study involved multiple reactions and reactants, such as N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9COA of Formula: C25H46ClN).

N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Aliphatic organochlorides are often alkylating agents as chlorine can act as a leaving group, which can result in cellular damage.COA of Formula: C25H46ClN

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Anti-inflammatory activity of CKC-containing cationic emulsion eye drop vehicles. was written by Daull, Philippe;Guenin, Samuel;Hamon de Almeida, Valérie;Garrigue, Jean-Sébastien. And the article was included in Molecular vision in 2018.COA of Formula: C25H46ClN The following contents are mentioned in the article:

Purpose: Preservative-free cationic emulsion-based artificial tears (ATs) or drug vehicles are innovative eye drop formulations with tear film stabilization and drug delivery properties, and valuable in vivo anti-inflammatory and wound healing properties. These ATs have recently reached the market as ATs for the management of dry eye disease (DED) symptoms (i.e., Cationorm) or as a drug vehicle for cyclosporine (Ikervis). The aim of the present study was to explore the mechanism of action underlying the intrinsic anti-inflammatory and wound-healing efficacies harbored by the cationic emulsions of cetalkonium chloride (CE-CKC). Methods: The anti-inflammatory activity of two CE-CKC (0.002% and 0.005% CKC) emulsions was evaluated by assessing the expression of proinflammatory genes and the secretion of various markers in the following human cell types stressed by different agents: peripheral blood mononuclear cells (PBMCs; stimulation with anti-CD3/anti-CD28 or lipopolysaccharide (LPS)), CD4⁺ T lymphocytes (TCD4; stimulation with anti-CD3/anti-CD28), and a human corneal epithelial cell line (HCE-2; stimulation with LPS). The cells were incubated for 30 min with a 10% dilution of CE-CKC emulsions and then cultured without the emulsions for 24 h or 72 h in the presence of the various challenging agents. The supernatant was collected, and the secreted markers quantitated with flow cytometry or an enzyme-linked immunosorbent assay (ELISA). Gene expression of inflammatory markers was evaluated only in the PBMCs and HCE-2 cells stimulated with LPS. The in vitro protein kinase C (PKC) binding assay for IC₅₀ determination was performed using standard procedures. Results: The CE-CKC emulsions decreased inflammatory gene expression in LPS-stimulated PBMCs (IFN-γ, IL-17A, CXCL-9, and TNFα) and LPS-stimulated HCE-2 cells (THBS1 and CCL2). Both CE-CKC emulsions inhibited the secretion of IL-17 (from anti-CD3/anti-CD28-stimulated TCD4), TNFα, IFN-γ, and IL-2 (from anti-CD3-/anti-CD28-stimulated PBMCs), and IL-6 and IL-8 (from LPS-stimulated HCE-2). The in vitro PKC binding assay revealed that CKC, the cationic agent, is a specific PKCα inhibitor. In addition, tyloxapol, another excipient, showed some anti-inflammatory activity on IL-6 and IL-8 in the LPS-stimulated HCE-2 cells. Conclusions: This study indicates that the CE-CKC emulsions are able to directly modulate the secretion and expression of proinflammatory cytokines and chemokines. The results also suggest that CKC and tyloxapol are pharmacologically active excipients with potentially beneficial effects in vivo. These data shed new light on the efficacy observed on the DED signs of these CE-CKC emulsions in clinical trials. This study involved multiple reactions and reactants, such as N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9COA of Formula: C25H46ClN).

N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9) belongs to organic chlorides. Organic chlorides are compounds containing a carbon-chlorine bond, which are widely used in the oil field as a wax dissolver. Aliphatic organochlorides are often alkylating agents as chlorine can act as a leaving group, which can result in cellular damage.COA of Formula: C25H46ClN

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Anti-inflammatory activity of CKC-containing cationic emulsion eye drop vehicles was written by Daull, Philippe;Guenin, Samuel;Hamon de Almeida, Valeie;Garrigue, Jean-Sebastien. And the article was included in Molecular Vision in 2018.Product Details of 122-18-9 The following contents are mentioned in the article:

Purpose: Preservative-free cationic emulsion-based artificial tears (ATs) or drug vehicles are innovative eye drop formulations with tear film stabilization and drug delivery properties, and valuable in vivo anti-inflammatory and wound healing properties. These ATs have recently reached the market as ATs for the management of dry eye disease (DED) symptoms (i.e., Cationorm) or as a drug vehicle for cyclosporine (Ikervis). The aim of the present study was to explore the mechanism of action underlying the intrinsic anti-inflammatory and wound-healing efficacies harbored by the cationic emulsions of cetalkonium chloride (CE-CKC). Methods: The anti-inflammatory activity of two CE-CKC (0.002% and 0.005% CKC) emulsions was evaluated by assessing the expression of proinflammatory genes and the secretion of various markers in the following human cell types stressed by different agents: peripheral blood mononuclear cells (PBMCs; stimulation with anti-CD3/anti-CD28 or lipopolysaccharide (LPS)), CD4+ T lymphocytes (TCD4; stimulation with anti-CD3/anti-CD28), and a human corneal epithelial cell line (HCE-2; stimulation with LPS). The cells were incubated for 30 min with a 10% dilution of CE-CKC emulsions and then cultured without the emulsions for 24 h or 72 h in the presence of the various challenging agents. The supernatant was collected, and the secreted markers quantitated with flow cytometry or an ELISA (ELISA). Gene expression of inflammatory markers was evaluated only in the PBMCs and HCE-2 cells stimulated with LPS. The in vitro protein kinase C (PKC) binding assay for IC50 determination was performed using standard procedures. Results: The CE-CKC emulsions decreased inflammatory gene expression in LPS-stimulated PBMCs (IFN-γ, IL-17A, CXCL-9, and TNFα) and LPS-stimulated HCE-2 cells (THBS1 and CCL2). Both CE-CKC emulsions inhibited the secretion of IL-17 (from anti-CD3/anti-CD28-stimulated TCD4), TNFα, IFN-γ, and IL-2 (from anti-CD3-/anti-CD28-stimulated PBMCs), and IL-6 and IL-8 (from LPS-stimulated HCE-2). The in vitro PKC binding assay revealed that CKC, the cationic agent, is a specific PKCα inhibitor. In addition, tyloxapol, another excipient, showed some anti-inflammatory activity on IL-6 and IL-8 in the LPS-stimulated HCE-2 cells. Conclusions: This study indicates that the CE-CKC emulsions are able to directly modulate the secretion and expression of proinflammatory cytokines and chemokines. The results also suggest that CKC and tyloxapol are pharmacol. active excipients with potentially beneficial effects in vivo. These data shed new light on the efficacy observed on the DED signs of these CE-CKC emulsions in clin. trials. This study involved multiple reactions and reactants, such as N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9Product Details of 122-18-9).

N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. The haloform reaction, using chlorine and sodium hydroxide, is also able to generate alkyl halides from methyl ketones, and related compounds. Chloroform was formerly produced thus.Product Details of 122-18-9

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Disinfectant and Antimicrobial Susceptibility Profiles of Campylobacter coli Isolated in 1998 to 1999 and 2015 from Swine and Commercial Pork Chops was written by Beier, Ross C.;Harvey, Roger B.;Hernandez, Charles A.;Andrews, Kathleen;Droleskey, Robert E.;Hume, Michael E.;Davidson, Maureen K.;Bodeis-Jones, Sonya;Young, Shenia;Anderson, Robin C.;Nisbet, David J.. And the article was included in Journal of Food Science in 2019.Electric Literature of C25H46ClN The following contents are mentioned in the article:

Susceptibility profiles were determined for 111 Campylobacter coli strains obtained in 1998 to 1999 and 2015 from market age pigs and pork chops against 22 disinfectants and 9 antimicrobials. Resistance to tetracycline (TET) was observed in 44.4% of 1998 to 1999 strains, and the antibiotic resistance profile was TET. But strains obtained in 2015 from swine and retail pork chops had 75% TET resistance and the antibiotic resistance profile was TET, followed by azithromycin-erythromycin-TET-telithromycin-clindamycin. Antimicrobial resistance increased in 2015 strains. All strains were resistant to triclosan, and 84.1% and 95.8% of strains in 1998 to 1999 and 2015, resp., were chlorhexidine resistant. All strains were susceptible to benzalkonium chloride. There was a shift toward higher susceptibility to chlorhexidine, triclosan, P-128, OdoBan, CPB, and CPC in 2015 swine and pork chop strains compared with 1998 to 1999 strains. The disinfectants Tek-Trol and providone-iodine, tris(hydroxylmethyl)nitromethane (THN) and formaldehyde demonstrated the highest susceptibilities. Didecyldimethylammonium chloride (C10AC) appeared to be about equally effective as benzyldimethyltetradecylammonium chloride (C14BAC) for inhibiting C. coli, and both were more effective than C8AC and C12BAC, but C16BAC was not efficient at inhibiting C. coli. The BACs, C12BAC and C14BAC, were the most effective ingredients in DC&R. Also, C12BAC and C14BAC, or these two in synergy with C10AC were responsible for inhibition of C. coli at high P-128 MICs. No cross-resistance was observed between antibiotics and disinfectants. The continued use of THN and formaldehyde in DC&R should be evaluated since these components are not effective, and their inclusion adds unwanted chems. in the environment. Practical Application : Campylobacter species cause diarrheal disease throughout the world. Disinfectants are often used on the farm, in veterinary medicine, by the food processing industry, in restaurants, and in consumer’s homes. Limited information is available in the literature showing how disinfectants or disinfectant components may affect the many different foodborne pathogens, and, specifically, Campylobacter coli studied here. The knowledge generated in this study concerning the interactions of a broad array of disinfectants against C. coli may well affect the types of disinfectants and disinfectant formulations allowable for use by medical personnel, producers, food processors, restaurants, and consumers. This study involved multiple reactions and reactants, such as N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9Electric Literature of C25H46ClN).

N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids. Alkyl chlorides readily react with amines to give substituted amines. Alkyl chlorides are substituted by softer halides such as the iodide in the Finkelstein reaction.Electric Literature of C25H46ClN

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

On May 22, 1951, Hayden, John J. published a patent.HPLC of Formula: 4569-86-2 The title of the patent was Azo safranine leather dyes. And the patent contained the following:

Azo dyes (I) of the safranine series for dyeing leather, particularly chrometanned leather, are obtained by coupling a monodiazo safranine compound with an N-(hydroxyalkyl)arylamine in which the N-hydroxyalkyl group contains at least 2 C atoms and which is unsubstituted in the 4-position. Thus, Methylene Violet 3RA diethyl analog paste 30 (containing 80% 3-amino-7-diethylamino-5-phenylphenazinium chloride) in concentrated H2SO4 16 is diazotized and coupled with PhN(CH2CH2OH)2 11 at pH 2.5-3.0 to give II (R and R’ = H), soluble in H2O, dyes chrome leather in bright greenish black shades. Similarly are obtained the following II (R and R’ given): Me, H, dyes brilliant dark green shades; AcNH, H, dyes brilliant dark green shades; N(CH2CH2OH)2, H, dyes brilliant, slightly reddish black shades; AcNH, MeO, dyes black shades; and 7-diethylamino-3-[4-(ethylhydroxyethylamino)phenylazo]-5-phenylphenazinium chloride. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).HPLC of Formula: 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. HPLC of Formula: 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

On March 7, 1951, Hayden, John J. published a patent.Recommanded Product: 4569-86-2 The title of the patent was Azo safranine leather dyes. And the patent contained the following:

Azo dyes (I) of the safranine series for dyeing leather, particularly chrometanned leather, are obtained by coupling a monodiazo safranine compound with an N-(hydroxyalkyl)arylamine in which the N-hydroxyalkyl group contains at least 2 C atoms and which is unsubstituted in the 4-position. Thus, Methylene Violet 3RA diethyl analog paste 30 (containing 80% 3-amino-7-diethylamino-5-phenylphenazinium chloride) in concentrated H2SO4 16 is diazotized and coupled with PhN(CH2CH2OH)2 11 at pH 2.5-3.0 to give II (R and R’ = H), soluble in H2O, dyes chrome leather in bright greenish black shades. Similarly are obtained the following II (R and R’ given): Me, H, dyes brilliant dark green shades; AcNH, H, dyes brilliant dark green shades; N(CH2CH2OH)2, H, dyes brilliant, slightly reddish black shades; AcNH, MeO, dyes black shades; and 7-diethylamino-3-[4-(ethylhydroxyethylamino)phenylazo]-5-phenylphenazinium chloride. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Recommanded Product: 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Recommanded Product: 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Hideo, Endo; Tada, Masao; Katagiri, Ken published an article in 1965, the title of the article was Antitumor activity of phenazine derivatives against S 180 and C 63 in mice I.SDS of cas: 4569-86-2 And the article contains the following content:

Phenazine and 68 derivatives of phenazine were screened for their antitumor activity against Sarcoma 180 and Carcinoma 63, transplanted in mice. The ratio of the average weights of tumors in the treated and the controls, and the ratio of body weight changes in the 2 groups were recorded for each compound 2-Methoxyphenazine, 3,7-diamino-2-methylphenazine and 3-morpholino-1,2-benzophenazine at doses of 50 mg./kg.-body weight/day, brought down the tumor weight to less than half in the carcinoma infected mice and 3-acetamido-7-amino-5-phenylphenazonium chloride at the same dosage caused a similar effect in the sarcoma-infected animals. No correlation was observed between tumor weight change and the change in body weight in the treated animals. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).SDS of cas: 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. SDS of cas: 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Cooperstein, S. J.; Lazarow, A.; Patterson, J. W. published an article in 1953, the title of the article was The mechanism of Janus Green B staining of mitochondria. II. Reactions and properties of Janus Green B and its derivatives.Application of 4569-86-2 And the article contains the following content:

The absorption spectra are reported of Janus Green B(I) and G(II) and their derivatives, diethylsafranine, leucosafranine, and dimethylsafranine. I was readily reduced by cysteine (III) and glutathione whereas II was not significantly reduced by III. At pH 7.48 I showed 2 reduction steps upon polarographic analysis, one at a potential of -0.001 v. and the other at -0.188 v. II had only one reduction step at -0.214 v. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Application of 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Application of 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Khan, Mohammad Rizwan; Khan, Moonis Ali; Alothman, Zeid Abdullah; Alsohaimi, Ibrahim Hotan; Naushad, Mu; Al-Shaalan, Nora H. published an article in 2014, the title of the article was Quantitative determination of methylene blue in environmental samples by solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry: a green approach.Safety of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride And the article contains the following content:

Industrial effluents with dyes may contain appreciable concentrations of materials with high COD and suspended solids, posing adverse effects to both humans and aquatic life; therefore, these effluents require quant. monitoring. In the present study, an anal. method based on solid-phase extraction (SPE) and ultra-performance liquid chromatog.-tandem mass spectrometry (UPLC-MS/MS) has been optimized for the quant. anal. of methylene blue (MB) in environmental samples. For the extraction of MB, a variety of solvents, including formic acid, were investigated to obtain optimum recovery. MB extraction using the SPE method was best achieved using methanol with 1 M formic acid. Chromatog. separation of MB and methylene violet 3RAX (MV 3RAX, internal standard) was accomplished on an Acquity BEH C18 column using water (64.99%) with formic acid (0.01%) and acetonitrile (35%) in isocratic elution mode. Chromatog. separation for both MB and MV 3RAX was achieved in <2 min with good resolution and superior peak symmetry. MB and MV 3RAX were quantified with electrospray ionization coupled with MS/MS in the multiple reaction monitoring mode. Good quality parameters were achieved for instance linearity (r2 > 0.999), run-to-run and day-to-day precisions with relative standard deviations of <4%, sensitivity with a low limit of detection (LOD) of 0.1 ng mL-1 and limit of quantification (LOQ) of 0.4 ng mL-1 were obtained. Industrial wastewater samples (paper, textile, laundry and printing press) were analyzed, and an MB level was found in between 0.36 and 1.08 μg mL-1 with excellent recovery rates (95-99%) depending on samples. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Safety of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

The Article related to environmental sample methylene blue spe uplc tandem mass spectrometry, Water: Analysis and other aspects.Safety of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics