On March 6, 2003, Ewing, Gregory; Mullah, Khairuzzaman; Graham, Ronald published a patent.Name: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride The title of the patent was Non-fluorescent bis-diazo triaryl and aryldiazo-N-arylphenazonium quencher compounds for use in biomolecular assays. And the patent contained the following:
Bis-diazo,triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomols. such as polynucleotides, nucleosides, nucleotides, and polypeptides. The quencher moieties are non-fluorescent and accept energy from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomols. for various mol. biol. assays based on fluorescence detection. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Name: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
The Article related to diazo triaryl arylphenazonium fluorescence quencher bioassay, Biochemical Methods: Spectral and Related Methods and other aspects.Name: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics