Category: 4569-86-2

On May 22, 1951, Hayden, John J. published a patent.HPLC of Formula: 4569-86-2 The title of the patent was Azo safranine leather dyes. And the patent contained the following:

Azo dyes (I) of the safranine series for dyeing leather, particularly chrometanned leather, are obtained by coupling a monodiazo safranine compound with an N-(hydroxyalkyl)arylamine in which the N-hydroxyalkyl group contains at least 2 C atoms and which is unsubstituted in the 4-position. Thus, Methylene Violet 3RA diethyl analog paste 30 (containing 80% 3-amino-7-diethylamino-5-phenylphenazinium chloride) in concentrated H2SO4 16 is diazotized and coupled with PhN(CH2CH2OH)2 11 at pH 2.5-3.0 to give II (R and R’ = H), soluble in H2O, dyes chrome leather in bright greenish black shades. Similarly are obtained the following II (R and R’ given): Me, H, dyes brilliant dark green shades; AcNH, H, dyes brilliant dark green shades; N(CH2CH2OH)2, H, dyes brilliant, slightly reddish black shades; AcNH, MeO, dyes black shades; and 7-diethylamino-3-[4-(ethylhydroxyethylamino)phenylazo]-5-phenylphenazinium chloride. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).HPLC of Formula: 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. HPLC of Formula: 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

On March 7, 1951, Hayden, John J. published a patent.Recommanded Product: 4569-86-2 The title of the patent was Azo safranine leather dyes. And the patent contained the following:

Azo dyes (I) of the safranine series for dyeing leather, particularly chrometanned leather, are obtained by coupling a monodiazo safranine compound with an N-(hydroxyalkyl)arylamine in which the N-hydroxyalkyl group contains at least 2 C atoms and which is unsubstituted in the 4-position. Thus, Methylene Violet 3RA diethyl analog paste 30 (containing 80% 3-amino-7-diethylamino-5-phenylphenazinium chloride) in concentrated H2SO4 16 is diazotized and coupled with PhN(CH2CH2OH)2 11 at pH 2.5-3.0 to give II (R and R’ = H), soluble in H2O, dyes chrome leather in bright greenish black shades. Similarly are obtained the following II (R and R’ given): Me, H, dyes brilliant dark green shades; AcNH, H, dyes brilliant dark green shades; N(CH2CH2OH)2, H, dyes brilliant, slightly reddish black shades; AcNH, MeO, dyes black shades; and 7-diethylamino-3-[4-(ethylhydroxyethylamino)phenylazo]-5-phenylphenazinium chloride. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Recommanded Product: 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Recommanded Product: 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Hideo, Endo; Tada, Masao; Katagiri, Ken published an article in 1965, the title of the article was Antitumor activity of phenazine derivatives against S 180 and C 63 in mice I.SDS of cas: 4569-86-2 And the article contains the following content:

Phenazine and 68 derivatives of phenazine were screened for their antitumor activity against Sarcoma 180 and Carcinoma 63, transplanted in mice. The ratio of the average weights of tumors in the treated and the controls, and the ratio of body weight changes in the 2 groups were recorded for each compound 2-Methoxyphenazine, 3,7-diamino-2-methylphenazine and 3-morpholino-1,2-benzophenazine at doses of 50 mg./kg.-body weight/day, brought down the tumor weight to less than half in the carcinoma infected mice and 3-acetamido-7-amino-5-phenylphenazonium chloride at the same dosage caused a similar effect in the sarcoma-infected animals. No correlation was observed between tumor weight change and the change in body weight in the treated animals. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).SDS of cas: 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. SDS of cas: 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Cooperstein, S. J.; Lazarow, A.; Patterson, J. W. published an article in 1953, the title of the article was The mechanism of Janus Green B staining of mitochondria. II. Reactions and properties of Janus Green B and its derivatives.Application of 4569-86-2 And the article contains the following content:

The absorption spectra are reported of Janus Green B(I) and G(II) and their derivatives, diethylsafranine, leucosafranine, and dimethylsafranine. I was readily reduced by cysteine (III) and glutathione whereas II was not significantly reduced by III. At pH 7.48 I showed 2 reduction steps upon polarographic analysis, one at a potential of -0.001 v. and the other at -0.188 v. II had only one reduction step at -0.214 v. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Application of 4569-86-2

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Application of 4569-86-2

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Khan, Mohammad Rizwan; Khan, Moonis Ali; Alothman, Zeid Abdullah; Alsohaimi, Ibrahim Hotan; Naushad, Mu; Al-Shaalan, Nora H. published an article in 2014, the title of the article was Quantitative determination of methylene blue in environmental samples by solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry: a green approach.Safety of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride And the article contains the following content:

Industrial effluents with dyes may contain appreciable concentrations of materials with high COD and suspended solids, posing adverse effects to both humans and aquatic life; therefore, these effluents require quant. monitoring. In the present study, an anal. method based on solid-phase extraction (SPE) and ultra-performance liquid chromatog.-tandem mass spectrometry (UPLC-MS/MS) has been optimized for the quant. anal. of methylene blue (MB) in environmental samples. For the extraction of MB, a variety of solvents, including formic acid, were investigated to obtain optimum recovery. MB extraction using the SPE method was best achieved using methanol with 1 M formic acid. Chromatog. separation of MB and methylene violet 3RAX (MV 3RAX, internal standard) was accomplished on an Acquity BEH C18 column using water (64.99%) with formic acid (0.01%) and acetonitrile (35%) in isocratic elution mode. Chromatog. separation for both MB and MV 3RAX was achieved in <2 min with good resolution and superior peak symmetry. MB and MV 3RAX were quantified with electrospray ionization coupled with MS/MS in the multiple reaction monitoring mode. Good quality parameters were achieved for instance linearity (r2 > 0.999), run-to-run and day-to-day precisions with relative standard deviations of <4%, sensitivity with a low limit of detection (LOD) of 0.1 ng mL-1 and limit of quantification (LOQ) of 0.4 ng mL-1 were obtained. Industrial wastewater samples (paper, textile, laundry and printing press) were analyzed, and an MB level was found in between 0.36 and 1.08 μg mL-1 with excellent recovery rates (95-99%) depending on samples. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Safety of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

The Article related to environmental sample methylene blue spe uplc tandem mass spectrometry, Water: Analysis and other aspects.Safety of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Budzikiewicz, Herbert; Eckau, H.; Inhoffen, Hans H. published an article in 1970, the title of the article was Photosynthesis in green plants III. Investigation of the chemical processes in Metzner’s model for photosynthesis.Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride And the article contains the following content:

Thin-layer chromatog. investigations of the Metzner photosynthesis system show that no diethylsafranine is formed by reaction of Ag with janus green (I). Volume and pH measurements with and without I and chlorophyll a show that Cl formed by photolysis of AgCl, reacts with H2O to give HCl and O and also with I to give decomposition products. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

The Article related to metzner photosynthesis, photosynthesis metzner, janus green photosynthesis, chlorophylls role: cat (catalyst use), uses (uses), photolysis catalysts, photosynthesis, biological and other aspects.Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Brenner, Sydney published an article in 1953, the title of the article was Supravital staining of mitochondria with phenosafranine dyes.Category: chlorides-buliding-blocks And the article contains the following content:

The more basic a phenosafranine (I) dye, the lower is the concentration of dye necessary for the supravital staining of mitochondria, as determined by studies on the supravital staining of human lymphocyte mitochondria with various sym. and unsym. I dyes. The dyes studied were in the order of increasing basicity of the terminal atoms (as determined by spectral measurements) and staining ability, I, dimethyl-I, diethyl-I, tetramethyl-I, diethyldimethyl-I, tetraethyl-I, and Janus green B. The results are discussed in relation to theories of supravital staining. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Category: chlorides-buliding-blocks

The Article related to lymphocytes, mitochondria, stains and staining, animal tissue and other aspects.Category: chlorides-buliding-blocks

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Sorokin, Helen P. published an article in 1956, the title of the article was Living cells of pea seedlings. I. Survey of vacuolar precipitates, mitochondria, and spherosomes.Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride And the article contains the following content:

cf. C.A. 50, 9533b. The topmost portion of the 3rd internode in etiolated 7-day-old pea stems contained a definitely outlined region where Janus Green B (I) was reduced to diethylsafranin. Some cells in this region, particularly those adjacent to the starch sheath, formed stable, copious, brilliant blue-green precipitates of A-type vacuoles. Combined staining with I and neutral red separated red precipitates from blue mitochondria. The latter were recognized by the reversible coloration and decolorization of I under aerobic and anaerobic conditions, resp. They became dark red when incubated with neotetrazolium chloride. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

On January 25, 2012, Honda, Aki; Suzuki, Koji published a patent.Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride The title of the patent was Method for determining allergen in environment, and simple allergen quantitation kit. And the patent contained the following:

An allergen determination method is provided, which enables to conveniently measuring the quantity of a biol. allergen (e.g., acari, pollen) in environment without using an anti-allergen antibody. The method comprises using as a substrate for a proteinase which the allergen possesses a substrate capable of bringing a visible color change as a result of an enzymic reaction, bringing a solution containing this substrate into contact with a measurement object material collected using an adhesive sheet, measuring the proteinase activity in the measurement object material using the color change in the substrate solution as an index, and thereby, determining the biol. allergen. The proteinase substrate used in this method is a coloring compound (e.g, Boc-Val-Leu-Lys-3RAX, Boc-amino acid-3RAX) in which an amino acid or an oligopeptide is bound to at least one amino group of a coloring dye possessing at least one amino group (e.g., cresyl violet, safranine O, methylene violet 3RAX). The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

The Article related to allergen environment determination proteinase coloring dye substrate colorimetry, Biochemical Methods: Biological and other aspects.Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

On December 21, 2006, Iwasaki, Aki; Suzuki, Koji published a patent.Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride The title of the patent was Method for determining allergen in environment, and simple allergen quantitation kit. And the patent contained the following:

An allergen determination method is provided, which enables to conveniently measuring the quantity of a biol. allergen (e.g., acari, pollen) in environment without using an anti-allergen antibody. The method comprises using as a substrate for a proteinase which the allergen possesses a substrate capable of bringing a visible color change as a result of an enzymic reaction, bringing a solution containing this substrate into contact with a measurement object material collected using an adhesive sheet, measuring the proteinase activity in the measurement object material using the color change in the substrate solution as an index, and thereby, determining the biol. allergen. The proteinase substrate used in this method is a coloring compound (e.g, Boc-Val-Leu-Lys-3RAX, Boc-amino acid-3RAX) in which an amino acid or an oligopeptide is bound to at least one amino group of a coloring dye possessing at least one amino group (e.g., cresyl violet, safranine O, methylene violet 3RAX). The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

The Article related to allergen environment determination proteinase coloring dye substrate colorimetry, Biochemical Methods: Biological and other aspects.Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics