CON4EI: Short Time Exposure (STE) test method for hazard identification and labelling of eye irritating chemicals was written by Adriaens, E.;Willoughby, J. A. Sr.;Meyer, B. R.;Blakeman, L. C.;Alepee, N.;Fochtman, P.;Guest, R.;Kandarova, H.;Verstraelen, S.;Van Rompay, A. R.. And the article was included in Toxicology In Vitro in 2018.Reference of 96568-04-6 This article mentions the following:
Assessment of ocular irritancy is an international regulatory requirement in the safety evaluation of industrial and consumer products. Although many in vitro ocular irritation assays exist, alone they are incapable of fully categorizing chems. Therefore, the CEFIC-LRI-AIMT6-VITO CON4EI consortium was developed to assess the reliability of eight in vitro test methods and establish an optimal tiered-testing strategy. One assay selected was the Short Time Exposure (STE) assay. This assay measures the viability of SIRC rabbit corneal cells after 5 min exposure to 5% and 0.05% solutions of test material, and is capable of categorizing of Category 1 and No Category chems. The accuracy of the STE test method to identify Cat 1 chems. was 61.3% with 23.7% sensitivity and 95.2% specificity. If non-soluble chems. and unqualified results were excluded, the performance to identify Cat 1 chems. remained similar (accuracy 62.2% with 22.7% sensitivity and 100% specificity). The accuracy of the STE test method to identify No Cat chems. was 72.5% with 66.2% sensitivity and 100% specificity. Excluding highly volatile chems., non-surfactant solids and non-qualified results resulted in an important improvement of the performance of the STE test method (accuracy 96.2% with 81.8% sensitivity and 100% specificity). Furthermore, it seems that solids are more difficult to test in the STE, 71.4% of the solids resulted in unqualified results (solubility issues and/or high variation between independent runs) whereas for liquids 13.2% of the results were not qualified, supporting the restriction of the test method regarding the testing of solids. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Reference of 96568-04-6).
Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. Alkyl chlorides are versatile building blocks in organic chemistry. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available.Reference of 96568-04-6
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics