A rapid and inexpensive genotyping method using dried blood spots for mutational analysis in a mutant mouse model: an update was written by Romano, Antonella;Zuchegna, Candida;Zannini, Giuseppa;Messina, Samantha;Fusco, Clorinda;Pezone, Antonio;Grillo, Roberta;de Candia, Paola;Matarese, Giuseppe;Porcellini, Antonio. And the article was included in Molecular Biology Reports in 2022.Recommanded Product: Lithium chloride The following contents are mentioned in the article:
Dried blood spot (DBS) testing is a well-known method of bio-sampling by which blood samples are blotted and dried on filter paper. The dried samples can then be analyzed by several techniques such as DNA amplification and HPLC. We have developed a non-invasive sampling followed by an alternative protocol for genomic DNA extraction from a drop of blood adsorbed on paper support. This protocol consists of two sep. steps: (1) organic DNA extraction from the DBS, followed by (2) DNA amplification by polymerase chain reaction (PCR). The PCR-restriction fragment length polymorphism (PCR-RFLP) is an advantageous and simple approach to detect single nucleotide polymorphisms (SNPs). We have evaluated the efficiency of our method for the extraction of genomic DNA from DBS by testing its performance in genotyping mouse models of obesity and herein discuss the specificity and feasibility of this novel procedure. Our protocol is easy to perform, fast and inexpensive and allows the isolation of pure DNA from a tiny amount of sample. This study involved multiple reactions and reactants, such as Lithium chloride (cas: 7447-41-8Recommanded Product: Lithium chloride).
Lithium chloride (cas: 7447-41-8) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Recommanded Product: Lithium chloride
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Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics