High performance liquid chromatography-based method to analyze activity of GABA transporters in central nervous system was written by Rogerio da Silva Moraes, Edinaldo;Santos-Silva, Mateus;Grisolia, Alan Araujo;Braga, Danielle Valente;Reis Leao, Luana Ketlen;Bahia, Carlomagno Pacheco;Soares de Moraes, Suellen Alessandra;Passos, Adelaide Fonseca;de Jesus Oliveira Batista, Evander;Herculano, Anderson Manoel;Matos Oliveira, Karen Renata Herculano. And the article was included in Neurochemistry International in 2022.Quality Control of Lithium chloride The following contents are mentioned in the article:
The GATs are the membrane proteins responsible for the uptake of GABA in the central nervous system. Alterations in GAT activity are implicated in several neurol. diseases, including retinopathies. The present study describes an alternative method to determine GAT activity in tissue preparations of the central nervous system, using high performance liquid chromatog. (HPLC) with fluorescence detection. The GABA concentration in the medium was determined using the o-phthaldehyde (OPA)-derivation protocol validated by the Brazilian Health Regulatory Agency (ANVISA) and the United States Food and Drug Administration (US-FDA). The GAT activity in the retinal preparations was determined through the evaluation of the GABA uptake, which was measured by assessing the difference between the initial and final concentrations of GABA in the incubation medium. The evaluation of the GAT kinetics returned values of Km = 382.5 ± 32.2μM and Vmax = 34 nmol/mg of protein. The data also demonstrated that the GABA uptake was predominantly Na+- and temperature-dependent, and was also inhibited by incubation with nipecotic acid, a substrate of GABA transporters. Taken together, these findings confirm that our approach provided a specific measure of GAT activity in retinal tissue. The data presented here thus validate, for the first time, an alternative, simple and sensitive method for the evaluation of GAT activity using high performance chromatog. on preparations of the central nervous system. This study involved multiple reactions and reactants, such as Lithium chloride (cas: 7447-41-8Quality Control of Lithium chloride).
Lithium chloride (cas: 7447-41-8) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids. The haloform reaction, using chlorine and sodium hydroxide, is also able to generate alkyl halides from methyl ketones, and related compounds. Chloroform was formerly produced thus.Quality Control of Lithium chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics