Synthesis, spectroscopic characterization, antimicrobial evaluation and molecular docking study of novel triazine-quinazolinone based hybrids was written by Dinari, Mohammad;Gharahi, Fateme;Asadi, Parvin. And the article was included in Journal of Molecular Structure in 2018.Synthetic Route of C9H6Cl2N4 The following contents are mentioned in the article:
A series of new 1,3,5-triazines incorporating aromatic quinazolinone moiety as potential antimicrobial agents was reported. The first chlorine group of the cyanuric chloride was replaced by aniline and the second one was replaced by various aromatic amines. The prepared monochlorotriazine was allowed to react with hydrazine to give hydrazinyl triazines I (R = Ph, 4-ClC6H4, 4-NCC6H4, etc.). These hydrazinyl triazines reacted with 2-methyl-4H-benzo[1,3]oxazin-4-one to give novel triazine-quinazolinone based hybrids II. The chem. structure and purity of the hybrid compounds were evaluated by different techniques such as thin layer chromatog., m.p., Fourier-transform IR (FTIR), 1H and 13C NMR spectra and elemental anal. Antimicrobial activity of the hybrid compounds was studied on three Gram-neg. bacteria (Salmonella enteritidis, E. coli, P. aeruginosa) and three Gram-pos. bacteria (S. aureus, Listeria monocytogenes, Bacillus subtilis) as well as Candida albicans as a yeast-like fungus using the serial broth dilution method. Among them, compound I (R = 4-H2NO2SC6H4) showed higher antimicrobial activity with a min. inhibitory concentration (MIC) value of 16 μg/mL. Furthermore, compounds I (R = 4-H2NO2SC6H4, 4-ClC6H4, 4-NCC6H4) showed good activity against several tested strains. In addition, docking simulation was performed to position best antibacterial compounds into the S. aureus dihydrofolate reductase (DHFR) active site to determine the probable binding conformations. This study involved multiple reactions and reactants, such as 4,6-Dichloro-N-phenyl-1,3,5-triazin-2-amine (cas: 2272-40-4Synthetic Route of C9H6Cl2N4).
4,6-Dichloro-N-phenyl-1,3,5-triazin-2-amine (cas: 2272-40-4) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. The haloform reaction, using chlorine and sodium hydroxide, is also able to generate alkyl halides from methyl ketones, and related compounds. Chloroform was formerly produced thus.Synthetic Route of C9H6Cl2N4
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics