Cooperstein, S. J.; Lazarow, A.; Patterson, J. W. published an article in 1953, the title of the article was The mechanism of Janus Green B staining of mitochondria. II. Reactions and properties of Janus Green B and its derivatives.Application of 4569-86-2 And the article contains the following content:
The absorption spectra are reported of Janus Green B(I) and G(II) and their derivatives, diethylsafranine, leucosafranine, and dimethylsafranine. I was readily reduced by cysteine (III) and glutathione whereas II was not significantly reduced by III. At pH 7.48 I showed 2 reduction steps upon polarographic analysis, one at a potential of -0.001 v. and the other at -0.188 v. II had only one reduction step at -0.214 v. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Application of 4569-86-2
3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Application of 4569-86-2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics