Sorokin, Helen P. published an article in 1956, the title of the article was Living cells of pea seedlings. I. Survey of vacuolar precipitates, mitochondria, and spherosomes.Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride And the article contains the following content:
cf. C.A. 50, 9533b. The topmost portion of the 3rd internode in etiolated 7-day-old pea stems contained a definitely outlined region where Janus Green B (I) was reduced to diethylsafranin. Some cells in this region, particularly those adjacent to the starch sheath, formed stable, copious, brilliant blue-green precipitates of A-type vacuoles. Combined staining with I and neutral red separated red precipitates from blue mitochondria. The latter were recognized by the reversible coloration and decolorization of I under aerobic and anaerobic conditions, resp. They became dark red when incubated with neotetrazolium chloride. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas:4569-86-2) belongs to chlorides. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst. Reference of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics