On December 21, 2006, Iwasaki, Aki; Suzuki, Koji published a patent.Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride The title of the patent was Method for determining allergen in environment, and simple allergen quantitation kit. And the patent contained the following:
An allergen determination method is provided, which enables to conveniently measuring the quantity of a biol. allergen (e.g., acari, pollen) in environment without using an anti-allergen antibody. The method comprises using as a substrate for a proteinase which the allergen possesses a substrate capable of bringing a visible color change as a result of an enzymic reaction, bringing a solution containing this substrate into contact with a measurement object material collected using an adhesive sheet, measuring the proteinase activity in the measurement object material using the color change in the substrate solution as an index, and thereby, determining the biol. allergen. The proteinase substrate used in this method is a coloring compound (e.g, Boc-Val-Leu-Lys-3RAX, Boc-amino acid-3RAX) in which an amino acid or an oligopeptide is bound to at least one amino group of a coloring dye possessing at least one amino group (e.g., cresyl violet, safranine O, methylene violet 3RAX). The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
The Article related to allergen environment determination proteinase coloring dye substrate colorimetry, Biochemical Methods: Biological and other aspects.Quality Control of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
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