On January 12, 2021, Hsu, Sheng-Hsin; Zhang, Shouqi; Huang, Sheng-Cih; Wu, Tung-Kung; Xu, Zhengren; Chang, Chin-Yuan published an article.Related Products of 98946-18-0 The title of the article was Characterization of enzymes catalyzing the formation of the nonproteinogenic amino acid L-Dap in capreomycin biosynthesis. And the article contained the following:
Capreomycin (CMN) and viomycin (VIO) are nonribosomal peptide antituberculosis antibiotics, the structures of which contain four nonproteinogenic amino acids, including L-2,3-diaminopropionic acid (L-Dap), β-ureidodehydroalanine, L-capreomycidine, and β-lysine. Previous bioinformatics anal. suggested that CmnB/VioB and CmnK/VioK participate in the formation of L-Dap; however, the real substrates of these enzymes are yet to be confirmed. We herein show that starting from O-phospho-L-Ser (OPS) and L-Glu precursors, CmnB catalyzes the condensation reaction to generate a metabolite intermediate N-(1-amino-1-carboxyl-2-ethyl)glutamic acid (ACEGA), which undergoes NAD+-dependent oxidative hydrolysis by CmnK to generate L-Dap. Furthermore, the binding site of ACEGA and the catalytic mechanism of CmnK were elucidated with the assistance of three crystal structures, including those of apo-CmnK, the NAD+-CmnK complex, and CmnK in an alternative conformation. The CmnK-ACEGA docking model revealed that the glutamate α-hydrogen points toward the nicotinamide moiety. It provides evidence that the reaction is dependent on hydride transfer to form an imine intermediate, which is subsequently hydrolyzed by a water mol. to produce L-Dap. These findings modify the original proposed pathway and provide insights into L-Dap formation in the biosynthesis of other related natural products. The experimental process involved the reaction of tert-Butyl trichloroacetimidate(cas: 98946-18-0).Related Products of 98946-18-0
The Article related to capreomycin biosynthesis amino acid diaminopropionic acid enzyme structure saccharothrix, Enzymes: Separation-Purification-General Characterization and other aspects.Related Products of 98946-18-0
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