Zhao, Jing; Jin, Yan; Shin, Yujin; Jeong, Kyung Min; Lee, Jeongmi published an article in 2016, the title of the article was Indirect enantioseparation of fluoxetine in mouse serum by derivatization with 1R-(-)-menthyl chloroformate followed by ultra high performance liquid chromatography and quadrupole time-of-flight mass spectrometry.Safety of (1R,2S,5R)-2-Isopropyl-5-methylcyclohexyl carbonochloridate And the article contains the following content:
Here we describe a simple and sensitive anal. method for the enantioselective quantification of fluoxetine in mouse serum using ultra high performance liquid chromatog. with quadrupole time-of-flight mass spectrometry. The sample preparation method included a simple deproteinization with acetonitrile in 50 μL of serum, followed by derivatization of the extracts in 50 μL of 2 mM 1R-(-)-menthyl chloroformate at 45 C for 55 min. These conditions were statistically optimized through response surface methodol. using a central composite design. Under the optimized conditions, neither racemization nor kinetic resolution occurred. The derivatized diastereomers were readily resolved on a conventional sub-2 μm C18 column under a simple gradient elution of aqueous methanol containing 0.1% formic acid. The established method was validated and found to be linear, precise, and accurate over the concentration range of 5.0-1000.0 ng/mL for both R and S enantiomers (r2 > 0.993). Stability tests of the prepared samples at three different concentration levels showed that the R- and S-fluoxetine derivatives were relatively stable for 48 h. No significant matrix effects were observed Last, the developed method was successfully used for enantiomeric anal. of real serum samples collected at a number of time points from mice administered with racemic fluoxetine. The experimental process involved the reaction of (1R,2S,5R)-2-Isopropyl-5-methylcyclohexyl carbonochloridate(cas: 14602-86-9).Safety of (1R,2S,5R)-2-Isopropyl-5-methylcyclohexyl carbonochloridate
The Article related to fluoxetine determination enantioseparation serum menthyl chloroformate uplc qtof ms, chiral derivatization reagent, enantioseparation, fluoxetine, menthyl chloroformate, response surface methodology and other aspects.Safety of (1R,2S,5R)-2-Isopropyl-5-methylcyclohexyl carbonochloridate
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