The author of 《Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching》 were Taylor, Nicole O.; Wei, Ming-Tzo; Stone, Howard A.; Brangwynne, Clifford P.. And the article was published in Biophysical Journal in 2019. Application of 7647-14-5 The author mentioned the following in the article:
Cells contain numerous membraneless organelles that assemble by intracellular liquid-liquid phase separation The viscous properties and associated biomol. mobility within these condensed phase droplets, or condensates, are increasingly recognized as important for cellular function and also dysfunction, for example, in protein aggregation pathologies. Fluorescence recovery after photobleaching (FRAP) is widely used to assess condensate fluidity and to estimate protein diffusion coefficients However, the models and assumptions utilized in FRAP anal. of protein condensates are often not carefully considered. Here, we combine FRAP experiments on both in vitro reconstituted droplets and intracellular condensates with systematic examination of different models that can be used to fit the data and evaluate the impact of model choice on measured values. A key finding is that model boundary conditions can give rise to widely divergent measured values. This has important implications, for example, in experiments that bleach subregions vs. the entire condensate, two commonly employed exptl. approaches. We suggest guidelines for determining the appropriate modeling framework and highlight emerging questions about the mol. dynamics at the droplet interface. The ability to accurately determine biomol. mobility both in the condensate interior and at the interface is important for obtaining quant. insights into condensate function, a key area for future research. In the experimental materials used by the author, we found Sodium chloride(cas: 7647-14-5Application of 7647-14-5)
Sodium chloride(cas: 7647-14-5) has been used for the preparation of tris buffered saline, phosphate buffered saline, MPM-2 (mitotic protein monoclonal 2) cell lysis buffer, immunoprecipitation wash buffer, LB (Luria-Bertani) media and dialysis buffer.Application of 7647-14-5
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics