Birgul, Kaan; Uba, Abdullah Ibrahim; Cuhadar, Ozan; Sevinc, Sevgi Kocyigit; Tiryaki, Selen; Tiber, Pinar Mega; Orun, Oya; Telci, Dilek; Yilmaz, Ozgur; Yelekci, Kemal; Guniz Kucukguzel, S. published an article in Journal of Molecular Structure. The title of the article was 《Synthesis and molecular modeling of MetAP2 of thiosemicarbazides, 1,2,4-triazoles, thioethers derived from (S)-Naproxen as possible breast cancer agents》.Reference of 3-Chlorobenzylchloride The author mentioned the following in the article:
New thiosemicarbazides, 1,2,4-triazoles and thioethers I (R1 = Ph, 4-FC6H4, Bn, etc.; R2 = Bn, 3-FC6H4CH2, 4-MeOC6H4CH2, etc.) from derived (S)-Naproxen were synthesized in this study. The mol. binding of the compounds on MetAP-2 was performed. Anticancer effects of the synthesized compounds were studied by using MTT assay method on MCF-7 (includes estrogen and progesterone receptors) and MDA-MB-231 (lacks estrogen and progesterone receptors) adenocarcinoma cell lines at 0, 10, 25, 50, 75 and 100μM concentrations for 24 h. The IC50 values of novel (S)-Naproxen derivatives were determined between from 5 to 100μM on MCF-7 breast cancer cell line and MDA-MB-231 cell lines. The apoptotic activity of selected compounds I (R1 = 4-ClC6H4; R2 = 3-FC6H4CH2) and I (R1 = Ph; R2 = 4-MeC6H4CH2) were first analyzed by Annexin V staining using Tali Image-Based Cytometer. Mitochondrial membrane potential changes determined in fluorescence plate reader following JC-1 stain for compounds I (R1 = 4-ClC6H4; R2 = 3-FC6H4CH2) and I (R1 = Ph; R2 = 4-MeC6H4CH2) in MCF-7 and MDA-MB-231 cells. The effect of these compounds on the cell viability 4T1 mouse mammary tumor cell line was tested at 1 to 5 times of calculated IC50 value (IC50x1, IC50x2, IC50x3, IC50x4, and IC50x5). Next in order to determine the toxicity of the combination of compound I (R1 = Bn; R2 = 3-FC6H4CH2) and Docetaxel, WST-1 cell viability and proliferation assay was performed with 4T1. The experimental process involved the reaction of 3-Chlorobenzylchloride(cas: 620-20-2Reference of 3-Chlorobenzylchloride)
3-Chlorobenzylchloride(cas: 620-20-2) has been used in the reaction of 3-methoxybenzyl chloride and ethyl 4-bromobenzoate in pure water, using zinc dust and a Pd catalyst.Reference of 3-Chlorobenzylchloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics