Finding of the low molecular weight inhibitors of resuscitation promoting factor enzymatic and resuscitation activity was written by Demina, Galina R.;Makarov, Vadim A.;Nikitushkin, Vadim D.;Ryabova, Olga B.;Vostroknutova, Galina N.;Salina, Elena G.;Shleeva, Margarita O.;Goncharenko, Anna V.;Kaprelyants, Arseny S.. And the article was included in PLoS One in 2009.Recommanded Product: 16588-16-2 This article mentions the following:
Background: Resuscitation promoting factors (RPF) are secreted proteins involved in reactivation of dormant actinobacteria, including Mycobacterium tuberculosis. They have been considered as prospective targets for the development of new anti-tuberculosis drugs preventing reactivation of dormant tubercle bacilli, generally associated with latent tuberculosis. However, no inhibitors of Rpf activity have been reported so far. The goal of this study was to find low mol. weight compounds inhibiting the enzymic and biol. activities of Rpfs. Methodol./Principal Findings: Here the authors describe a novel class of 2-nitrophenylthiocyanates (NPT) compounds that inhibit muralytic activity of Rpfs with IC50 1-7 μg/mL. Fluorescence studies revealed interaction of active NPTs with the internal regions of the Rpf mol. Candidate inhibitors of Rpf enzymic activity showed a bacteriostatic effect on growth of Micrococcus luteus (in which Rpf is essential for growth protein) at concentrations close to IC50. The candidate compounds suppressed resuscitation of dormant (“non-culturable”) cells of M. smegmatis at 1 μg/mL or delayed resuscitation of dormant M. tuberculosis obtained in laboratory conditions at 10 μg/mL. However, they did not inhibit growth of active mycobacteria under these concentrations Conclusions/Significance: NPT are the first example of low mol. weight compounds that inhibit the enzymic and biol. activities of Rpf proteins. In the experiment, the researchers used many compounds, for example, Ethyl 4-chloro-3-nitrobenzoate (cas: 16588-16-2Recommanded Product: 16588-16-2).
Ethyl 4-chloro-3-nitrobenzoate (cas: 16588-16-2) belongs to organic chlorides. Chlorination modifies the physical properties of hydrocarbons in several ways. These compounds are typically denser than water due to the higher atomic weight of chlorine versus hydrogen. Alkyl chlorides are versatile building blocks in organic chemistry. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available.Recommanded Product: 16588-16-2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics