Enzymatic hydrolytic resolution of (R,S)-α-chlorophenyl acetates in biphasic media was written by Wang, Pei-Yun;Chen, Teh-Liang;Tsai, Shau-Wei. And the article was included in Journal of Molecular Catalysis B: Enzymatic in 2007.Recommanded Product: Methyl 2-chloro-2-phenylacetate This article mentions the following:
A thermally stable carboxyl esterase (SNSM-87) from Klebsiella oxytoca is explored as an enantioselective biocatalyst for the hydrolytic resolution of (R,S)-α-chlorophenyl acetates in biphasic media, where the Et ester possessing the highest enantioselectivity (E* = 95) is selected as the best substrate and rationalized from the linear free energy relationships in terms of the logarithms of kinetic constants and enantiomeric ratio varied with the inductive parameter of leaving alc. An expanded Michaelis-Menten mechanism for the rate-limiting acylation step is adopted for the kinetic anal. Two-stage Bronsted slopes of 58.0 and 2.28 for the fast-reacting (R)-esters, as well as only one-stage slope of 4.15 for the slow-reacting (S)-esters, were found and elucidated from the change of rate-limiting step. The replacement of α-chloro substituent to α-Me or α-hydroxy moiety indicates that the α-substituent has profound effects on varying the enzyme activity, enantioselectivity, and optical-preference for SNSM-87 and lipases of pCPL, Lipase MY, NOVO 435 and Lipase PS. In the experiment, the researchers used many compounds, for example, Methyl 2-chloro-2-phenylacetate (cas: 7476-66-6Recommanded Product: Methyl 2-chloro-2-phenylacetate).
Methyl 2-chloro-2-phenylacetate (cas: 7476-66-6) belongs to organic chlorides. Organochlorines are organic compounds having multiple chlorine atoms. They were the first synthetic pesticides that were used in agriculture. They are resistant to most microbial and chemical degradations. Alkyl chlorides readily react with amines to give substituted amines. Alkyl chlorides are substituted by softer halides such as the iodide in the Finkelstein reaction.Recommanded Product: Methyl 2-chloro-2-phenylacetate
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics