Construction of a ratiometric electrochemical aptasensor based on graphdiyne-methylene blue and Fc-labeled hairpin for cyclic signal amplification detection of kanamycin was written by Gao, Xiaolin;Sun, Zhicong;Wang, Xiaoyang;Zhang, Wanqi;Wang, Yue;Han, Jie;Sun, Xia;Guo, Yemin;Li, Falan;Xu, Shicai. And the article was included in Sensors and Actuators, B: Chemical in 2022.Reference of 61-73-4 This article mentions the following:
Based on ferrocene (Fc)-labeled primers and graphdiyne-methylene blue (GDY-MB) nanocomposite, a ratiometric electrochem. aptasensor was prepared for high-precision detection of kanamycin (KAN). The Fc-labeled primer hybridized with the aptamer forms a rigid structure. When KAN was specifically bound to an aptamer, a primer with a self-complementary sequence was released to form a hairpin structure (HP), drawing the Fc close to the electrode surface, resulting in enhanced IFc. Exonuclease I (Exo I) was used to cleave the single-stranded aptamer and release the target, effectively increasing the cyclic amplification signal. The phosphorylation was carried out at the 3 end of the primers to prevent HP cleavage. The chitosan – antimony tin oxide (CS-ATO) and AuNPs were used to further modified the electrodes to double the signal amplification. Under the optimal exptl. conditions, the KAN concentration exhibited a strong linear relationship with IFc/IMB. The R2 (0.997) of the calibration curve was greater than the R2 (0.960) of the standard curve of KAN concentration and IFc, which indicates the robustness of the ratiometric electrochem. sensor. The limit of detection (LOD) was 6.044 nM. The aptasensor exhibits strong stability and specificity for successfully detection of KAN in real milk samples. In the experiment, the researchers used many compounds, for example, 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4Reference of 61-73-4).
3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Alkyl chlorides are versatile building blocks in organic chemistry. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available.Reference of 61-73-4
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics