Synthesis and identification of a new class of (S)-2,6-diamino-4,5,6,7-tetrahydrobenzo[d]thiazole derivatives as potent antileukemic agents was written by Prasanna, D. S.;Kavitha, C. V.;Raghava, B.;Vinaya, K.;Ranganatha, S. R.;Raghavan, Sathees C.;Rangappa, K. S.. And the article was included in Investigational New Drugs in 2010.SDS of cas: 6590-96-1 This article mentions the following:
Benzothiazoles are multitarget agents with broad spectrum of biol. activity. Among the antitumor agents discovered in recent years, the identification of various 2-(4-aminophenyl) benzothiazoles as potent and selective antitumor drugs against different cancer cell lines has stimulated remarkable interest. Some of the benzothiazoles are known to induce cell cycle arrest, activation of caspases and interaction with DNA mol. Based on these interesting properties of benzothiazoles and to obtain new biol. active agents, a series of novel 4,5,6,7-tetrahydrobenzo[d]thiazole derivatives, e.g. I, were synthesized and evaluated for their efficacy as antileukemic agents in human leukemia cells (K562 and Reh). The chem. structures of the synthesized compounds were confirmed by 1H NMR, LCMS and IR anal. The cytotoxicity of these compounds were determined using trypan blue exclusion, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Results showed that, these compounds mediate a significant cytotoxic response to cancer cell lines tested. It was found that the compounds having electron withdrawing groups at different positions of the Ph ring of the thiourea moiety displayed significant cytotoxic effect with IC50 value less than 60 μM. To rationalize the role of electron withdrawing group in the induction of cytotoxicity, I (IC50 â?5 μM) which is having chloro substitution at ortho and para positions was chosen . Flow cytometric anal. of annexin V-FITC/ propidium iodide (PI) double staining and DNA fragmentation suggest that I can induce apoptosis. In the experiment, the researchers used many compounds, for example, 2,4-Dichlorophenylisothiocyanate (cas: 6590-96-1SDS of cas: 6590-96-1).
2,4-Dichlorophenylisothiocyanate (cas: 6590-96-1) belongs to organic chlorides. Organic chlorides are compounds containing a carbon-chlorine bond, which are widely used in the oil field as a wax dissolver. Organochlorine compounds are lipophylic, meaning they are more soluble in fat than in water. This gives them a high tenancy to accumulate in the food chain (biomagnification).SDS of cas: 6590-96-1
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics