Design, synthesis and biological evaluation of a novel tubulin inhibitor SKLB0565 targeting the colchicine binding site was written by Hu, Xi;Li, Lu;Zhang, Qiangsheng;Wang, Qianqian;Feng, Zhanzhan;Xu, Ying;Xia, Yong;Yu, Luoting. And the article was included in Bioorganic Chemistry in 2020.Application In Synthesis of 2,6-Dichloro-9-isopropyl-9H-purine This article mentions the following:
A series of 3-(((9H-purin-6-yl)amino)methyl)pyridin-2(1H)-one derivatives were designed, synthesized and confirmed as tubulin polymerization inhibitors. All compounds were evaluated for their anti-proliferative activities on three colorectal carcinoma (CRC) cell lines. Among these compounds, SKLB0565 displayed noteworthy potency against eight CRC cell lines with IC50 values ranging from 0.012μM and 0.081μM. Besides, SKLB0565 inhibited tubulin polymerization, caused G2/M phase cell cycle arrest, depolarized mitochondria and induced cell apoptosis in CRC cells. Furthermore, SKLB0565 suppressed cell migration and disrupted the capillary tube formation of human umbilical vein endothelial cells (HUVECs). In this SKLB0565 is a promising anti-tubulin agent for CRC therapy which is worthy of further evaluation is clarified. In the experiment, the researchers used many compounds, for example, 2,6-Dichloro-9-isopropyl-9H-purine (cas: 203436-45-7Application In Synthesis of 2,6-Dichloro-9-isopropyl-9H-purine).
2,6-Dichloro-9-isopropyl-9H-purine (cas: 203436-45-7) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Application In Synthesis of 2,6-Dichloro-9-isopropyl-9H-purine
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics