Detection of stx2 from Shiga toxin-producing Escherichia coli (STEC) by a surface enhanced Raman spectroscopy (SERS) sensor using recycled silicon chips was written by Yang, Yuqing;Wasiewska, Luiza Adela;Burgess, Catherine M.;Duffy, Geraldine;Lovera, Pierre;O’Riordan, Alan. And the article was included in Sensors and Actuators, B: Chemical in 2022.Safety of 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride This article mentions the following:
In this research, a selective, cost-efficient, and highly sensitive Ag nanostructure Surface Enhanced Raman Spectroscopy sensor was developed as a methodol. approach to rapidly detect a targetss-DNA (stx2) in STEC (Shiga toxin-producing Escherichia coli). The Ag nanostructure-based SERS substrate was functionalized by two types of thiols: thiol-ss-DNA for bonding target ss-DNA and 6-Mercapto-1-hexanol for blocking the Ag nanostructure surface. Methylene Blue (MB) was used as a Raman marker to quantify target ss-DNA, as well as a model mol. to characterize the electrodeposited Ag nanostructure SERS substrate. Ag nanostructure SERS substrates showed good sensitivity and repeatability towards MB detection, with a LOD = 0.3158μM, and RSD = 12.48% (at 45 different random points for 0.1μM MB). More importantly, the Ag nanostructure/ss-DNA SERS substrate showed good selectivity towards STEC O157 stx2 target DNA, as well as good linearity and sensitivity towards its detection in a buffer solution The SERS sensors were able to identify target DNA (stx2) in a STEC strain and the study showed proof of principle that SERS substrate has potential as a cost-effective, highly selective, highly sensitive DNA and bacteria sensor without the aid of DNA amplification. With further development and validation, this methodol. approach has the potential for point-of-use detection for instance on a farm or in the food industry. In the experiment, the researchers used many compounds, for example, 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4Safety of 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride).
3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. The haloform reaction, using chlorine and sodium hydroxide, is also able to generate alkyl halides from methyl ketones, and related compounds. Chloroform was formerly produced thus.Safety of 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics