A photoaffinity displacement assay and probes to study the cyclin-dependent kinase family was written by Grant, Emma K.;Fallon, David J.;Eberl, H. Christian;Fantom, Ken G. M.;Zappacosta, Francesca;Messenger, Cassie;Tomkinson, Nicholas C. O.;Bush, Jacob T.. And the article was included in Angewandte Chemie, International Edition in 2019.Safety of 2,6-Dichloro-9-isopropyl-9H-purine This article mentions the following:
The CDK family plays a crucial role in the control of the cell cycle. Dysregulation and mutation of the CDKs has been implicated in cancer and the CDKs have been investigated extensively as potential therapeutic targets. Selective inhibition of specific isoforms of the CDKs is crucial to achieve therapeutic effect while minimizing toxicity. We present a group of photoaffinity probes designed to bind to the family of CDKs. The site of crosslinking of the optimized probe, as well as its ability to enrich members of the CDK family from cell lysates, was investigated. In a proof of concept study, we subsequently developed a photoaffinity probe-based competition assay to profile CDK inhibitors. We anticipate that this approach will be widely applicable to the study of small mol. binding to protein families of interest. In the experiment, the researchers used many compounds, for example, 2,6-Dichloro-9-isopropyl-9H-purine (cas: 203436-45-7Safety of 2,6-Dichloro-9-isopropyl-9H-purine).
2,6-Dichloro-9-isopropyl-9H-purine (cas: 203436-45-7) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Alkyl chlorides readily react with amines to give substituted amines. Alkyl chlorides are substituted by softer halides such as the iodide in the Finkelstein reaction.Safety of 2,6-Dichloro-9-isopropyl-9H-purine
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Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics