A small molecule inhibitor selective for a variant ATP-binding site of the chaperonin GroEL was written by Chapman, Eli;Farr, George W.;Furtak, Krystyna;Horwich, Arthur L.. And the article was included in Bioorganic & Medicinal Chemistry Letters in 2009.Formula: C9H9ClO2 This article mentions the following:
The chaperonin GroEL is a megadalton-sized mol. machine that plays an essential role in the bacterial cell assisting protein folding to the native state through actions requiring ATP binding and hydrolysis. A combination of medicinal chem. and genetics has been employed to generate an orthogonal pair, a small mol. that selectively inhibits ATPase activity of a GroEL ATP-binding pocket variant. An initial screen of kinase-directed inhibitors identified an active pyrazolo-pyrimidine scaffold that was iteratively modified and screened against a collective of GroEL nucleotide pocket variants to identify a cyclopentyl carboxamide derivative, EC3016, that specifically inhibits ATPase activity and protein folding by the GroEL mutant, I493C, involving a side chain positioned near the base of ATP. This orthogonal pair will enable in vitro studies of the action of ATP in triggering activation of GroEL-mediated protein folding and might enable further studies of GroEL action in vivo. The approach originated for studying kinases by Shokat and his colleagues may thus also be used to study large macromol. machines. In the experiment, the researchers used many compounds, for example, 2-(3-Methoxyphenyl)acetyl chloride (cas: 6834-42-0Formula: C9H9ClO2).
2-(3-Methoxyphenyl)acetyl chloride (cas: 6834-42-0) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Formula: C9H9ClO2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics