Harrill, Joshua A. published the artcileHigh-throughput transcriptomics platform for screening environmental chemicals, HPLC of Formula: 637-07-0, the publication is Toxicological Sciences (2021), 181(1), 68-89, database is CAplus and MEDLINE.
New approach methodologies (NAMs) that efficiently provide information about chem. hazard without using whole animals are needed to accelerate the pace of chem. risk assessments. Technol. advancements in gene expression assays have made in vitro high-throughput transcriptomics (HTTr) a feasible option for NAMs-based hazard characterization of environmental chems. In this study, we evaluated the Templated Oligo with Sequencing Readout (TempO-Seq) assay for HTTr concentration-response screening of a small set of chems. in the human-derived MCF7 cell model. Our exptl. design included a variety of reference samples and reference chem. treatments in order to objectively evaluate TempO-Seq assay performance. To facilitate anal. of these data, we developed a robust and scalable bioinformatics pipeline using open-source tools. We also developed a novel gene expression signature-based concentration-response modeling approach and compared the results to a previously implemented workflow for concentration-response anal. of transcriptomics data using BMDExpress. Anal. of reference samples and reference chem. treatments demonstrated highly reproducible differential gene expression signatures. In addition, we found that aggregating signals from individual genes into gene signatures prior to concentration-response modeling yielded in vitro transcriptional biol. pathway altering concentrations (BPACs) that were closely aligned with previous ToxCast high-throughput screening assays. Often these identified signatures were associated with the known mol. target of the chems. in our test set as the most sensitive components of the overall transcriptional response. This work has resulted in a novel and scalable in vitro HTTr workflow that is suitable for high-throughput hazard evaluation of environmental chems.
Toxicological Sciences published new progress about 637-07-0. 637-07-0 belongs to chlorides-buliding-blocks, auxiliary class Inhibitor,Cell Cycle,PPAR, name is Ethyl 2-(4-chlorophenoxy)-2-methylpropanoate, and the molecular formula is C12H15ClO3, HPLC of Formula: 637-07-0.
Referemce:
https://en.wikipedia.org/wiki/Chloride,
Chlorides – an overview | ScienceDirect Topics