De Pascale, G. published the artcileValidation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors, Application of N-Benzyl-N,N-dibutylbutan-1-aminium chloride, the publication is Journal of Applied Microbiology (2007), 103(1), 133-140, database is CAplus and MEDLINE.
The present study was undertaken to validate, for antibiotic discovery, a reporter gene assay based on a Bacillus subtilis strain expressing the Enterococcus faecium vanRS genes and a vanH-lacZ fusion, which produced β-galactosidase activity in the presence of cell wall inhibitors (CWI) and lysozyme. The reporter assay was miniaturized, automated and validated with antibiotics and tested against portions of chem. and microbial extract libraries. The assay is simple, fast and reproducible and can detect all CWI, sometimes at concentrations lower than those necessary to inhibit bacterial growth. However, some membrane-interfering compounds also generate comparable signals. While most CWI elicit a signal that is transcription-dependent and abolished in an osmoprotective medium, transcription is not required for β-galactosidase activity brought about by the membrane-interfering compounds At least two distinct mechanisms appear to lead to enzymic activity in the reporter strain. Effective counterscreens can be designed to discard the undesired classes of compounds Extensive validation is required before introducing a reporter assay in high-throughput screening. However, the ease of operation and manipulation makes the reporter assays powerful tools for antibiotic discovery.
Journal of Applied Microbiology published new progress about 23616-79-7. 23616-79-7 belongs to chlorides-buliding-blocks, auxiliary class Phase Transfer Catalyst, name is N-Benzyl-N,N-dibutylbutan-1-aminium chloride, and the molecular formula is C19H34ClN, Application of N-Benzyl-N,N-dibutylbutan-1-aminium chloride.
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