Biosynthesis of α-Substituted β-Ketoesters via the Tandem Knoevenagel Condensation-Reduction Reaction Using a Single Enzyme was written by Liu, Xiaolong;Li, Xiangjie;Wang, Zhelun;Zhou, Jinlong;Fan, Xinjiong;Fu, Yao. And the article was included in ACS Sustainable Chemistry & Engineering in 2020.Computed Properties of C6H9ClO3 The following contents are mentioned in the article:
Saturated α-substituted β ketoesters are important building blocks in synthesis of pharmaceuticals and agrochems. Herein, we report a one-pot biosynthesis of α-substituted β-ketoesters via Knoevenagel condensation and reduction of received unsaturated alkenes in situ, catalyzed by single ene-reductase (NerA). A series of inexpensive and readily available aldehydes and 1,3-diketones were condensed and reduced by NerA in aqueous solution at room temperature We also note that low loadings (3 mg/mL) of NerA are sufficient to facilitate the cascade process, both E and Z isomeric intermediates can be reduced effectively and improved the overall yield up to 95%. Meanwhile, the method can be applied in preparative-scale synthesis of pharmaceutical intermediate. This process conforms to the concepts of green chem. and shows advantages for synthesis of high value saturated α-substituted β ketoesters. This study involved multiple reactions and reactants, such as Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3Computed Properties of C6H9ClO3).
Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3) belongs to organic chlorides. Organic chlorides can cause corrosion in pipelines, valves and condensers, and cause catalyst poisoning. The hydrocarbon processing industry (HPI) and others are affected by damage caused by these substances. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Computed Properties of C6H9ClO3
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics