Highly efficient synthesis of pharmaceutically relevant chiral 3-N-substituted-azacyclic alcohols using two enantiocomplementary short chain dehydrogenases was written by Yang, Lin;Zhang, Yiping;Liu, Yan;Wang, Hualei;Wei, Dongzhi. And the article was included in Biochemical Engineering Journal in 2022.Related Products of 638-07-3 The following contents are mentioned in the article:
Two stereocomplementary alc. dehydrogenases from Flavobacterium psychrophilum (FpADH) and Flavobacterium sp. was reported. (FsADH), which showed the potential industrial application in highly efficient synthesis of a series of enantiomerically pure 3-N-substituted-azacyclic alochols. Both the enzymes showed high catalytic activity toward the model substrate N-Boc-4-piperidone (NBPO) and presented a strict enantioselectivity for the corresponding alc. products. In addition, both enzymes showed broad substrate scope, including ketoesters, acryl ketones and heterocyclic ketones. Using glucose dehydrogenase coexpressed with each of the enzymes to realize the efficient coenzyme recycling, various pharmaceutically relevant chiral 3-N-Boc azacyclic alcs. were asym. synthesized at high substrate concentrations (343.7-643.8 g/L) and low equilvelent of NADP+ (0.1 mM) with excellent enantioselectivity (> 99.5% e.e), which have met the requirements of biocatalytic processes in the industry and demonstrated the feasibility of FpADH and FsADH for industrial application in the biotransformation of chiral 3-N-substituted-azacyclic alcs. The mol. basis of the enantioselectivity and catalytic efficiency of both enzymes were revealed the by mol. docking and MD simulation anal. This study involved multiple reactions and reactants, such as Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3Related Products of 638-07-3).
Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. The haloform reaction, using chlorine and sodium hydroxide, is also able to generate alkyl halides from methyl ketones, and related compounds. Chloroform was formerly produced thus.Related Products of 638-07-3
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics