Chemoenzymatic catalysis of tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate by aldo-keto reductase coupled with composite Fe3O4 nanozyme scaffold was written by Qiu, Shuai;Xu, Shen-Yuan;Wang, Ya-Jun;Zheng, Yu-Guo. And the article was included in Chemical Engineering Science in 2022.Electric Literature of C6H9ClO3 The following contents are mentioned in the article:
Cofactor NADPH is widely used in enzymic redox reactions, however, its regeneration by glucose dehydrogenase (GDH) suffers from massive glucose consumption, costly wastewater treatment, and low atom efficiency. Herein, Fe3O4 nanoparticles supported on helical multi-walled carbon nanotubes (Fe3O4@HMWCNTs) was prepared and displayed comparable transfer hydrogenation activity to Exiguobacterium sibiricum GDH (EsGDH). We developed a chemoenzymic catalysis based on a combination of an aldo-keto reductase KmAKR with Fe3O4@HMWCNTs scaffold, which asym. synthesized optically pure tert-Bu 6-cyano-(3R,5R)-dihydroxyhexanoate ((3R,5R)-2, > 99.5% dep). Under the optimized conditions, 10 mM NADP+ was converted to NADPH by 5.0 mg Fe3O4@HMWCNTs, in a yield of > 80.0%. The combination of Fe3O4@HMWCNTs with KmAKRM7 completely reduced 10 mM tert-Bu 6-cyano-(5R)-hydroxy-3-oxohexanoate ((5R)-1) to (3R,5R)-2, in a conversion of > 99.0% and dep > 99.5%. Contrast to the previous developed biocatalytic approach with KmAKR and EsGDH, this efficient and compatible nanozyme-enzyme process required neither glucose/alcs. as co-substrate nor a glucose dehydrogenase/alc. dehydrogenase for NADPH regeneration. This study involved multiple reactions and reactants, such as Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3Electric Literature of C6H9ClO3).
Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3) belongs to organic chlorides. Organochlorines stimulate the central nervous system and cause convulsions, tremor, nausea, and mental confusion. Examples are dichlorodiphenyltrichloroethane (DDT), chlordane, lindane, endosulfan, and dieldrin. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Electric Literature of C6H9ClO3
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics