New ionic liquid increase the catalytic efficiency of recombinant Escherichia coli cells-mediated asymmetric reduction was written by Li, Jun;Fan, Miaoliang;Zhang, Rongjin;Tian, Zheyu;Zeng, Jing;Song, Yinan;Du, Wenting. And the article was included in Journal of Chemical Technology and Biotechnology in 2019.Safety of Ethyl 4-chloro-3-oxobutanoate The following contents are mentioned in the article:
Background : Ionic liquids (ILs) have advantages in terms of flexible structure and composition for the whole cell mediated biocatalytic reaction. However, the lack of design strategy for ILs has hampered the development of biocatalysis in non-aqueous medium. In the present study, to establish an efficient reaction system to obtain vital chiral compounds, a novel IL was designed by combining the superior characteristics of quaternary ammonium- and dialkyl-based cationic surfactant IL to enhance the recombinant Escherichia coli cells-catalyzed bioreduction efficiency. Results : [HOEtN1,1,1][BF4] was synthesized and showed excellent characteristics of low toxicity to the biocatalyst, reduced toxicity of substrate to the microbial cells and moderate increase of cell membrane permeability. In the [HOEtN1,1,1][BF4]- containing reaction system, some valuable factors involved in the biocatalytic reduction of Et 4-chloro-3-oxobutanoate to Et (S)-4-chloro-3-hydroxybutanoate were found to be: 2.5% (w/v) [HOEtN1,1,1][BF4], 20% (volume/volume) isopropanol, 3 M COBE, pH 7.0, 30°C, reacted for 16 h. A yield of 96.7% was obtained under the optimum conditions with above 99% enantiomeric excess value, and the proposed bioprocess was also successfully scaled up to a 5 L fermentor with a yield of 90.7%. Conclusion : The developed IL [HOEtN1,1,1][BF4] is a promising solvent for enhancing the efficiency of model bioreduction It was also evaluated for broadening its application to the biosynthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol catalyzed by recombinant Escherichia coli, with remarkable success. © 2018 Society of Chem. Industry. This study involved multiple reactions and reactants, such as Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3Safety of Ethyl 4-chloro-3-oxobutanoate).
Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids.While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available. Alkyl chlorides readily undergo attack by nucleophiles.Safety of Ethyl 4-chloro-3-oxobutanoate
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics