Efficient profiling of detergent-assisted membrane proteome in cyanobacteria was written by Choi, Jong-Soon;Park, Yun Hwan;Oh, Jeong Hyun;Kim, Sooyong;Kwon, Joseph;Choi, Yoon-E.. And the article was included in Journal of Applied Phycology in 2020.Reference of 122-18-9 The following contents are mentioned in the article:
Membrane proteins play key roles in cellular functions like transport of mols., perception of environmental cues, and signal transduction into the intracellular compartment. However, the profiling of membrane proteins is still a daunting task because of the hydrophobicity, restricting our knowledge of membrane proteins. Thus, we attempted to develop a novel detergent-based approach to uncover membrane proteins using cyanobacteria. We investigated the effect of five different detergents on the profiling of the cyanobacterial membrane proteome. The application of either amidosulfobetaine-14 (ASB14) or N-lauroylsarcosine (NL) doubled the number of the identified integral membrane proteins compared with the control. Extraction with ASB14 increased the number of transmembrane helixes over four times. The quant. index (mol%) of membrane proteins also increased from 13 to 22% when ASB14, NL, and benzyldimethyl-n-hexadecylammonium chloride (BAC) were used. ASB14 treatment was particularly useful for identifying membrane proteins with higher mol. weights (Mr), and the addition of BAC to the cyanobacterial membrane could identify the membrane proteins with acidic isoelec. points (pI). To validate the efficiency of the detergent-based proteomics, the functional membrane protein complexes involved in energy metabolism were selected as an example and shown to be successful with the combined data of ASB14, NL, and zwittergent 3-10 (ZW3-10). Taken together, we suggest that the use of a specific detergent and the subsequent combination of proteome data is critical for detailed profiling of cyanobacterial functional membrane proteins. This study involved multiple reactions and reactants, such as N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9Reference of 122-18-9).
N-Benzyl-N,N-dimethylhexadecan-1-aminium chloride (cas: 122-18-9) belongs to organic chlorides. Chlorination modifies the physical properties of hydrocarbons in several ways. These compounds are typically denser than water due to the higher atomic weight of chlorine versus hydrogen. Aliphatic organochlorides are often alkylating agents as chlorine can act as a leaving group, which can result in cellular damage.Reference of 122-18-9
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics