On November 15, 2001, Cook, Ronald M.; Lyttle, Matt; Dick, Daren published a patent.SDS of cas: 4569-86-2 The title of the patent was Synthesis and methods for dark quencher probes for donor-acceptor energy transfer. And the patent contained the following:
The invention concerns a family of quenchers of excited state energy that are substantially non-fluorescent, termed “”Black Hole Quenchers”” (BHQs). The quenchers of the invention remedy many of the deficiencies of currently utilized dark quenchers, probes assembled using these quenchers and methods using such quenchers and probes. Further, the dark quenchers are functionalized to allow their rapid attachment to probe that can be engineered to have a desired light absorption profile. The provision of this class of dark quenchers represents a substantial improvement in the design of probes incorporating dark quenchers and methods using such probes. Also provided are methods of using the BHQs, synthesis of such probes incorporating the BHQs and methods of using the probes. The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).SDS of cas: 4569-86-2
The Article related to fret black hole quencher probe nucleic acid fluorophore, Biochemical Methods: Synthesis and other aspects.SDS of cas: 4569-86-2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics