On June 29, 2006, Honda, Aki; Suzuki, Koji published a patent.Name: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride The title of the patent was Proteinase activity-measuring method/reagent using colored dye-modified peptide substrate. And the patent contained the following:
A proteinase activity-measuring method/reagent is provided, which enables to conveniently measure a proteinase activity without requiring a special equipment. The method for measuring a proteinase activity in a test sample comprises performing an enzymic reaction using as a substrate for the proteinase in the test sample a colored substance formed by binding through an amide bond an amino acid or an oligopeptide with at least one amino group in the amino groups of a colored dye (e.g., cresyl violet, safranine O, methylene violet 3RAX) possessing at least one amino group, and measuring the proteinase activity based on a color change of the colored compound The experimental process involved the reaction of 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride(cas: 4569-86-2).Name: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
The Article related to proteinase assay reagent colored dye peptide substrate, Enzymes: Analysis (Determination-Detection) and other aspects.Name: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics