Kim, Hyeon-Young; Kim, Tae Rim; Kim, Sung-Hwan; Kim, In-Hyeon; Ko, Youngho; Yun, Sungil; Lee, In-Chul; Park, Han-Oh; Kim, Jong-Choon published the artcile< Genotoxicity evaluation of self-assembled-micelle inhibitory RNA-targeting amphiregulin (SAMiRNA-AREG), a novel siRNA nanoparticle for the treatment of fibrotic disease>, HPLC of Formula: 6055-19-2, the main research area is amphiregulin SAMiRNA targeting nanoparticle fibrotic disease treatment genotoxicity; GLP study; Self-assembled-micelle interfering RNA nanoparticle; amphiregulin; genotoxicity; safety evaluation.
The self-assembled-micelle inhibitory RNA-targeting amphiregulin (SAMiRNA-AREG) is a novel small-interfering RNA (siRNA) nanoparticle that is used for treatment of pulmonary fibrosis. We investigated the potential genotoxicity of SAMiRNA-AREG based on the guidelines published by the Organization for Economic Cooperation and Development. In the bacterial reverse mutation assay (Ames test), SAMiRNA-AREG did not induce mutations in Salmonella typhimurium TA100, TA1535, TA98, and TA1537 and Escherichia coli WP2uvrA at concentrations of up to 3000μg/plate with or without metabolic activation. The SAMiRNA-AREG (concentrations up to 500μg/mL) did not induce chromosomal aberrations in cultured Chinese hamster lung cells with or without metabolic activation. In the in vivo mouse bone marrow micronucleus assay, the SAMiRNA-AREG (concentrations up to 300 mg/kg body weight) did not affect the proportions of polychromatic erythrocytes and total erythrocytes, nor did it increase the number of micronucleated polychromatic erythrocytes in ICR mice. Collectively, these results suggest that SAMiRNA-AREG is safe with regard to genotoxicity such as mutagenesis or clastogenesis under the present exptl. conditions. These results might support the safety of SAMiRNA-AREG as a potential therapeutic agent for pharmaceutical development.
Drug and Chemical Toxicology (1977) published new progress about Ames test. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, HPLC of Formula: 6055-19-2.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics