Svalova, Tatiana S.; Medvedeva, Margarita V.; Saigushkina, Anna A.; Kozitsin, Ivan V.; Malysheva, Natalya N.; Zhdanovskikh, Valeria O.; Okhokhonin, A. V.; Kozitsina, Alisa N. published the artcile< A label-free impedimetric immunosensor based on covalent immobilization of anti-E. Coli antibody via a copper-catalyzed azide-alkyne cycloaddition reaction>, COA of Formula: C9H9Cl, the main research area is impedimetry immunosensor antibody immobilization; Antibody covalent immobilization; Copper-catalyzed azide-alkyne cycloaddition; E. coli; Label-free impedimetric immunosensor.
An original method is proposed for address and covalent immobilization of anti-E. coli antibodies on a screen-printed electrode (SPE). The method is based on a copper-catalyzed “”click”” reaction between a polyvinylbenzylazide (PVBA) film electrochem. deposited on the electrode surface and acetylene fragments of propargyl-N-hydroxysuccinimide ester. The products of electrochem. oxidation of copper particles incorporated in the polymer film on the electrode were first used for catalysis of the click reaction. This approach allowed the authors to reduce the immobilization time from a few hours for conventional methods to just 30 min, and to prevent denaturation of the immunoreceptor. The modified electrodes were characterized by SEM, electrochem. impedance spectroscopy (EIS), and cyclic voltammetry (CV). Based on the results obtained, a label-free impedimetric immunosensor for E. coli detection was developed. The detection limit of the immunosensor was estimated as 6.3 CFU/mL, with a linear range of 103-106 CFU/mL. The immunosensor demonstrated good stability during 30 days of storage in phosphate buffer solution (PBS, pH 7) and selectivity toward excess Staphylococcus aureus bacteria.
Analytical and Bioanalytical Chemistry published new progress about Antibodies and Immunoglobulins Role: ARG (Analytical Reagent Use), BUU (Biological Use, Unclassified), TEM (Technical or Engineered Material Use), ANST (Analytical Study), USES (Uses), BIOL (Biological Study) (anti-E.coli). 1592-20-7 belongs to class chlorides-buliding-blocks, and the molecular formula is C9H9Cl, COA of Formula: C9H9Cl.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics