An, Ning; Gong, Pisheng; Hou, Henglei; Chi, Weiya; Jin, Haibo; Zhao, Lan; Tan, Qiqi; Tang, Xu; Wang, Fei; Jin, Hongchao; Zhang, Rongyue published the artcile< Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus>, Application In Synthesis of 1592-20-7, the main research area is macroporous microsphere core shell structure chromatog purification virus; Avian influenza virus; Macroporous microsphere with core-shell structure; Negative chromatography; Purification of virus.
The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through neg. chromatog. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with neg. charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the com. available resin (12 mg/mL, Capto Core 700).
Journal of Chromatography A published new progress about Chromatographic stationary phases. 1592-20-7 belongs to class chlorides-buliding-blocks, and the molecular formula is C9H9Cl, Application In Synthesis of 1592-20-7.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics