Kotova, N.; Hebert, N.; Haernwall, E.-L.; Vare, D.; Mazurier, C.; Douay, L.; Jenssen, D.; Grawe, J. published the artcile< A novel micronucleus in vitro assay utilizing human hematopoietic stem cells>, Product Details of C7H17Cl2N2O3P, the main research area is micronucleus assay human erythrocyte hematopoietic stem cell genotoxicity; Erythrocytes; Flow cytometry; Genotoxicity in vitro; Micronucleus test; Stem cells.
The induction of micronucleated reticulocytes in the bone marrow is a sensitive indicator of chromosomal damage. Therefore, the micronucleus assay in rodents is widely used in genotoxicity and carcinogenicity testing. A test system based on cultured human primary cells could potentially provide better prediction compared to animal tests, increasing patient safety while also implementing the 3Rs principle, i.e., replace, reduce and refine. Hereby, the authors describe the development of an in vitro micronucleus assay based on animal-free ex vivo culture of human red blood cells from hematopoietic stem cells. To validate the method, five clastogens with direct action, three clastogens requiring metabolic activation, four aneugenic and three non-genotoxic compounds have been tested. Also, different metabolic systems have been applied. Flow cytometry was used for detection and enumeration of micronuclei. Altogether, the results were in agreement with the published data and indicated that a sensitive and cost effective in vitro assay to assess genotoxicity with a potential to high-throughput screening has been developed.
Toxicology In Vitro published new progress about Aneugens. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, Product Details of C7H17Cl2N2O3P.
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