Fischer, A.; Koeper, L. M.; Vohr, H.-W. published the artcile< Specific antibody responses of primary cells from different cell sources are able to predict immunotoxicity in vitro>, Quality Control of 6055-19-2, the main research area is immunotoxicity test antibody spleen PBMC cell.
Alternative methods for the prediction of immunotoxicity are highly desirable. However, until now no in vitro test for this purpose has been fully validated or accepted by regulatory authorities. MD cultures are in vitro equivalent to the widely used ex vivo primary T cell dependent antibody responses (TDAR), which has been identified in a regulatory context as a main functional test for immunotoxicol. investigations. The purpose of the present study was to use MD cultures of spleen and blood cells to compare data from three different chems. using SRBC as antigen in two different species. Using this approach we were able to show that cell sources from both rats and mice were able to correctly predict all tested compounds and to clearly distinguish immunosuppressants from control substances. Furthermore, animal studies can be refined by using MD cultures of PBMC. During a 28d benzo(a)pyrene treatment of rats we were able to follow the kinetic of an immune response by in vitro analyses. Addnl. evaluation of in vitro antibody responses of spleen cells and PBMC from rats treated with cyclophosphamide revealed similar results compared to the conventional ex vivo plaque forming cell assay (PFCA). In conclusion, investigation of in vitro antibody responses is a sensitive and reliable approach for detection of a compound induced specific effect on the immune system. MD cultures may not only replace the ex vivo TDAR in the future, but their implementation in routine toxicol. also enables refinement of existing in vivo studies by reducing the numbers of animals.
Toxicology In Vitro published new progress about Antibodies and Immunoglobulins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, Quality Control of 6055-19-2.
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Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics